Project 19

RGC dendritic morphology in ephrin-A-/- mice

Ephrin-As are expressed in retinal ganglion cells and have been detected in their dendrites as well as their axons. This project will investigate whether ephrin-As are involved in regulating the morphology of RGC dendrites and their connections within the retina. Single RGCs from normal and ephrin-A-/- mice will be filled with dye to reveal their dendritic processes and axon trajectories within the retina. Methods: Single cell injection with fluorescent dye, immunohistochemistry, image analysis and neurolucida software.