Project 22

Axon regeneration induced by genetically modified Schwann cells

Using viral vectors it is possible to introduce growth factor genes into retinal ganglion cells (RGC) and into Schwann cells. We have shown previously that Schwann cells genetically modified to express CNTF can be incorporated into peripheral nerve sheaths and, when grafted onto the injured optic nerve, they promote increased regeneration of adult RGC axons. In this project we will examine whether similar types of graft also promote the regeneration of RGC axons when the injury occurs in the brain, at a significant distance from the eye. RGCs may also be modified using appropriate viral vectors. The study address a critical issue in neural repair - the extent to which the site of axon injury affects the regenerative capability of an injured nerve cell.