Project 5

Behavioural characterisation of ephrin-A2/ephrin-A5 double knockout mice: focus on vision and memory.

The project addresses the role of Eph/ephrin interactions in the development of visual system connectivity and their contribution to remodelling synaptic connections in the adult during learning and memory. There is considerable evidence that Eph/ephrin interactions play an important role in both processes. Double knockout mice for two key members of the ephrin-A family, ephrin-A2 and ephrin-A5 provides an exceptional model in which to carry out behavioural studies.

The honours project will address a selection of the aims listed below, depending of the areas of interest of the student. The project will involve working closely with the animal care unit (maintaining breeding records) and regular genotyping of the animals.

1. Behavioural studies:

General characterization of knockout mice: reflexes, motor skills, sensory function

Learning and memory: radial arm maze

Visual function: visual acuity, optokinetic response, orienting behaviour, light entrainment of circadian rhythms.

2. Anatomical and physiological studies:

Retinal ganglion cell counts

Retinal axon decussation patterns

Electrophysiological recording of retino-collicular topography