Project 17

Neural Therapeutics: The effects of a neuroprotective molecule on retinal structure and the retinal vasculature after optic nerve crush in rodents

We have recently shown that, after cutting all retinal ganglion cell axons in adult rat, intraocular injections of a neuroprotective molecule under patent significantly enhances retinal ganglion cell survival compared to saline injected controls. Furthermore, the neuroprotective molecule induces the regeneration of some retinal ganglion cell axons beyond the injury site. However, possible side effects have yet to be assessed. In this project, we will determine whether the neuroprotective molecule has any effects on cells other than retinal ganglion cells. A variety of immunohistochemical markers will be used in both retinal wholemounts and sections and tissue examined using fluorescence and confocal microscopy. Specifically, we will examine a number of different cell types which normally act as sentinels for impending damage. For example, GFAP and vimentin will be used to determine the reactive status of Muller cells which are known to be highly reactive to toxic insult. The lectin Griffonia simplicifolia will be used to examine the retinal vasculature since this is known to be particularly susceptible to pathological change. Rod photoreceptors are also extremely vulnerable and will be examined using rhodopsin antibodies.